Thy1-gcamp6s mice
Webb16 apr. 2024 · Because multiple ACX areas contribute to auditory processing, we first performed WF imaging of GCaMP6s in awake mice. For unbiased identification of ACX areas, we developed an automated image segmentation algorithm based upon temporal responses. We detected known and other ACX areas. Webb20 nov. 2024 · A-TG mice exhibited Aβ pathology in the hippocampus. In several configurations of an air-induced running task, A-TG mice and C-TG mice were equally …
Thy1-gcamp6s mice
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WebbCortical imaging confirmed that type 1 expressed the cortical GCaMP6s, while type 2 did not. Conclusion: These results suggest that observation of the cross-sectioned tail in Thy1-GCaMP6s mice enabled to identify the phenotype within approximately 10 min/mouse, which reduces the cost and time for genotyping. Webb7 jan. 2024 · In this study, we describe an experimental procedure for measuring calcium dynamics through dual optical windows above bilateral primary somatosensory …
Webb25 feb. 2024 · Transgenic Thy1-GCaMP6s mice were administered three doses of propofol during P7–P11 and assessed at 4 months of age. Immunostaining in the motor cortex revealed a 10–18% reduction in the density of NeuN + cells and GCaMP6 + pyramidal neurones in both L2/3 and L5 of the motor cortex after repeated propofol exposure ( Fig. … Webb6 apr. 2024 · We had previously shown that THY1 (CD90) is a tumor suppressor in nasopharyngeal carcinoma (NPC) and that its down-regulation and loss of expression are associated with tumor metastasis, yet the mechanism leading to such effects remains unknown. In this study we show that tumor invasion could be suppressed by THY1 via …
Webb4 apr. 2024 · We implanted the optical window into GP4.3 (Thy1-GCaMP6s) mice at E15.5 and observed neurotransmission among three cells, as shown in movie S5 (Fig. 2, ,I I and andJ J). The placenta is a barrier that impedes passage of chemicals such as drugs into the embryo, but the diffusion rates for molecules are difficult to assess ( 28 , 29 ). Webb15 mars 2024 · Here we describe five new transgenic mouse lines expressing GCaMP6F (fast) or GCaMP6S (slow) in the central and peripheral nervous system under the control of the Thy1.2 promoter. Results These transgenic lines exhibit stable and layer-specific expression of GCaMP6 in multiple brain regions.
Webb1 mars 2024 · Thy1 -GCaMP6s mice (Jackson labs stock number 024275) were used in a subset of auditory brainstem response (ABR) measurements. Imaging experiments were performed on male and female offspring of Thy1 -GCaMP6 crossed with CBA/CaJ, a strain that retains good ABR thresholds through adulthood ( Zheng et al. 1999 ).
Webb024276 C57BL/6J-Tg(Thy1-GCaMP6f)GP5.5Dkim/J These transgenic mice, made by the Genetically-Encoded Neuronal Indicator and Effector ( GENIE ) Project, express the green … low sodium meals ready to eatWebbTo circumvent this problem, we crossed the Thy1-GCaMP6s line to the CBA/CaJ line, which retains excellent hearing into adulthood, and confirmed that high-frequency ABR thresholds in Thy1-GCaMP6s × CBA mice were lower than the Thy1-GCaMP6s at all ages (Wilcoxon rank-sum test, P < 0.0005 for all ages) and did not change through 20 weeks ... low sodium meat choicesWebb18 okt. 2012 · In Thy1-GCaMP2.2c mice, we observed GCaMP-expressing neurons but could not detect activated cells within an imaging window of 250 × 250 μm during a 10 … low sodium meatloaf mixWebb24 sep. 2014 · Here we present the generation and characterization of transgenic mice expressing the GECIs GCaMP6s or GCaMP6f under the Thy1 promoter. We quantified GCaMP6 expression across brain regions... low sodium meatloafWebb13 maj 2024 · Mice were maintained in a temperature-controlled environment at Columbia University Medical Center with ad libitum access to food and water. Adult C57BL/6 mice of both sexes (8 to 12 wk old) were purchased from Jackson Laboratory. Transgenic Thy1.2-GCaMP6s mice expressing GCaMP6 slow in some DRG neurons have been previously … low sodium meatloaf allrecipesWebbCommon Name: GP4.3. These transgenic mice, made by the Genetically-Encoded Neuronal Indicator and Effector ( GENIE) Project, express the green fluorescent calcium indicator, … jaydayoungan they miss the old meWebb23 jan. 2024 · Specifically, we injected into the S1 of Vip IRES-Cre; Thy1-GCaMP6s mice with Cre-dependent mRuby2-P2A-GCaMP6s and AAV2-retro (a variant of AAV2 permitting robust retrograde access to projection ... jaydayoungan the real jumpman 23 download