2024 Phis2-p53

Phis2-p53

Author: tuny

August undefined, 2024

http://www.bnbiotech.com/beinuobio-Products-11104093/ http://www.biofeng.com/zaiti/jiaomuzajiao/pHis2.html

酵母杂交实验常见问题——单杂知识及自激活检测篇_元件_-bait_作用

http://www.qualityard.com/upload/2024-12-17/dbd8dacfb1f8d0626e1c10bc4840ba05.pdf Webb30 okt. 2024 · Vectors pHIS2, p53-HIS2 + pGAD-Rec-53 and pMutant-HIS2 + pGADT7-TcMYC were the blank, positive, and negative controls, respectively. Transformed yeast cells were dropped onto a selective medium containing synthetic dextrose (SD) without leucine, tryptophan, and histidine but with 10–50 mM 3-aminotriazole (SD/-Leu/-Trp/-His … receiving seeds in the mail from china

Identification and characterization of MYC transcription …

http://www.bnbiotech.com/beinuobio-Products-11104093/ Webbp53-His,pLacZ,pHis，pHisi-1. 1、长期保存的储存菌株以冷冻干燥保藏为主，特殊要求的菌种可用其它方法进行保存，保存期可长达几年至几十年。. 2、半储存菌株以含20%甘油的肉汤或液体石腊封存的半固体琼脂为主，在0℃以下保存，保存期为3－6个月。. 3、应用菌株 … WebbPlasmid Phis2 1 Cloning Reporter Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article … receiving sensory input

VvMYB14 participates in melatonin-induced proanthocyanidin …

Webb实验步骤: 1.构建载体目的基因融合连入pGADT7-Rec2 (AD); 顺式作用元件连入pHis2或pHis2.1载体 (顺式元件可以只连接一个拷贝，也可以连接3个串联的并测序确认无误)。. 2.验证两载体分别于对应的空载体共转时，有无激活转录报告基因情况，大多酵母苗株有毒 … Webb18 juli 2006 · We generated yeast one-hybrid reporter plasmid by cloning the 356-bp p53 promoter fragment into the pHIS2 reporter plasmid (pHIS2-p53 prom.) and then cotransformed the yeast strain Y187 cells with pHIS2-p53-prom. reporter plasmid and pGAD-Rec2-p53 encoding p53-GAL4 AD fusion. We used pHIS2-p53 cons. reporter … receiving serverWebb9 apr. 2024 · +pHIS2-p53 3 1.2 酵母转化方法 pGAD53m +pHIS2-p53 1. 从YPDA平板挑取Y187单菌落接种于YPDA液体培养基4 ml，30℃，225 rpm，振荡培养18-20 h（过 … receiving shaft

"Webb3 mars 2024 · The pHIS2-p53/pGADT7-p53 and pHIS2-p53/pGADT7-PsnSHN2 were used as the positive and negative control, respectively. 1, 10 −2 and 10 −4 represent solution dilution ratio of transformed Y187 ... " - Phis2-p53

Phis2-p53

Multi-scale modeling identifies the role of p53-Gys2

Webb27 apr. 2024 · The glycogen synthase 2 (Gys2) and p53 generates a novel negative feedback circuit in which p53 represses Gys2 expression whereas Gys2 can stabilize … Webb24 jan. 2024 · The p53 fragment was from pGBKT7-p53 used for Y2H. ... The promoter cloned in pHIS2.1 is strong autoactivator and the yeast colonies transformed with this is growing even at 100mM 3-AT.

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Webb9 apr. 2024 · 目的：发现与A相互作用的motif 方法：以构建到pGADT7载体上的A基因为诱饵，筛选酵母单杂交motif文库，经过对阳性克隆的基因检测、DNA测序和比对分析，确定与pGADT7-A相互作用的motif。材料： 1．诱饵克隆：pGADT7-A 2．诱饵载体：pGADT7 3．文库酵母质粒：motif 4．Clontech酵母单杂交系统 5．培养基 Webb产品名称. p53-His,pLacZ,pHis，pHisi-1. 商品货号. B80888. 中文名称. 酵母单杂交. 冻干物说明. ·冻干首次活化,干粉要全部用完,不能保留,用0.2-0.5m1的培养液或者无菌水溶解,接种 …

WebbFRFS-0353-2 For Epcos saw filter P/N B4959 REV 1.00 Electro-Photonics LLC reserves the right to modify drawings, configurations, specifications, and design at any time without … Webb14 aug. 2015 · zt028 酵母单杂交载体系统 pHis2 Clontech ... zt1220 pGBK-p53 . zt1221 pGC-silencer-U6-GFP . zt1222 pGIPZ . zt1223 pGL3-FOXO-reporter-luci . zt1224 pGL4.13 . zt1225 pGL4.18 . zt1226 pGL4.19 . zt1227 pGL4.74 . zt1228 pGL5luci . zt1229 ...

Webb4 okt. 2013 · The interactions of pHIS2-p53 (three tandem copies of the cis-acting DNA consensus sequence in pHIS2, which is recognized by p53) with the tested TFs were used as negative controls. Two MYC ( ThMYC4 and ThMYC6 GenBank number: JN166788 and JN166790) were identified to bind to the E-box motif, of which ThMYC6 that bound with … http://www.biofeng.com/zaiti/jiaomu/p53his.html

Webb6 sep. 2024 · 1）pHis2-bait和AD-prey质粒混合作为实验组，将pHis2-bait和空载AD质粒混合作为自激活组。将实验组和自激活组分别共转到Y187感受态细胞中。 2）用SD/-Leu/ …

WebbEngineered Process receiving sheetWebbp53-His2. Catalog No. PVT11251 Packing . p53-His2 Information. Function yeast Hybrid plasmid Resistance Kan Screen / Strain DH5alpha Culture temperature 37degrees … univ.-prof. dr. timon adolph phdWebbpHis2: 质粒类型: 酵母单杂交载体: 高拷贝/低拷贝:--启动子:--克隆方法: 多克隆位点，限制性内切酶: 载体大小: 7207bp: 5' 测序引物及序列:--3' 测序引物及序列:--载体标签: TRP1, HIS3: … univrse interactive sdn bhdhttp://www.engineeredprocess.com/images/Honeywell_IFM_P531.P532_IOM.pdf univrm unknown shaderWebb5 apr. 2024 · The Y187 strain co-expressing pGADT7-LkARF7 and pHIS2-LkBBM1 and the strain co-expressing pGADT7-LkARF19 and pHIS2-LkBBM1 grew normally on TDO medium containing 75 mM 3-AT, ... The bait vectors pGBKT7-p53 and pGBKT7-LaminC were co-transformed into AH109 with the prey vector pGADT7-LargeT as positive and negative … receiving shares as payment for servicesWebb7 dec. 2024 · For Y1H assays, sequences including the MBS or ERE elements from the promoter of VvMYBPA1 or VvMYBPA2 were cloned into the pHis2 vector. Mutant MBS or ERE elements were used as a negative control. The ORF of VvMYB14 or VvERF104 was cloned into the pGADT7 vector. receiving shipWebbBait pHIS2.1 containing repeats of your target sequence of interest Prey pGADT7-Rec2 vector containing a library gene Yeast Phenotypes His–, Leu–, or Trp– Requires histidine (His), leucine (Leu), or tryptophan (Trp) in the medium to grow; i.e., is auxotrophic for one (or more) of these specific nutrients Miscellaneous receiving shares from employer