Phee401e vector
WebJul 21, 2015 · Arabidopsis mutants produced by constitutive overexpression of the CRISPR/Cas9 genome editing system are usually mosaics in the T1 generation. In this study, we used egg cell-specific promoters to drive the expression of Cas9 and obtained non-mosaic T1 mutants for multiple target genes with high efficiency. Comparisons of 12 … WebOct 24, 2024 · Two sgRNAs were designed and cloned into pHEE401E as described by Wang et al. (2015b). ... China) for the CRISPR/Cas9 vector; Xiao-Su Gao, Jiqin Li, Yun-Xiao He, and Shui-Ning Yang (Chinese Academy of Sciences Center for Excellence in Molecular Plant Sciences, China) for skillful technical assistance; Hongtao Liu, Lin Xu (Chinese Academy …
Phee401e vector
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WebNov 22, 2024 · The pHEE401E_UBQ_Bar vector is a version in which the egg-specific promoter for Cas9 expression in the pHEE401E vector was replaced with the UBQ10 … WebAug 13, 2024 · EAR (Ethylene-responsive element binding factor-associated Amphiphilic Repression) motif-containing transcription repressors have been shown to regulate plant growth and development, and plant responses to plant hormones and environmental stresses including biotic and abiotic stresses. However, the functions of most EAR-motif …
WebJun 22, 2024 · An mCherry gene under the control of a seed-specific At2S3 promoter was cloned into the plasmid pHEE401E to generate pHEE401E-mCherry 14,16. ... we used the … WebJan 17, 2024 · pHEE401E binary vector. pHEE401E is based on pCambia backbone for Agrobacterium-mediated transformation. It contains the complementary parts of the …
WebNov 22, 2024 · The pHEE401E vector has a type IIS restriction-enzyme-recognition site for ligation [6]. The other is a pBAtC-tRNA vector that uses a polycistronic tRNA-gRNA expression system and has two AarI type IIS restriction enzyme sites. Originally, these two vectors could carry two sgRNAs. WebThe construct is split into one module vector set (pCBC-DT1T2) containing two single guide RNAs (sgRNAs) and a binary vector based on pCAMBIA (pHEE401E) containing additional …
WebNov 22, 2024 · The vectors used were pHEE401E_UBQ_Bar and pBAtC_tRNA, which employ a one-promoter/one-sgRNA and a polycistronic-tRNA-gRNA strategy, respectively. Golden Gate cloning was performed with type IIS...
WebJan 7, 2024 · pHEE401E article : bacterial resistance : Kanamycin cloning : growth notes : Please see the cloning protocol attachments for detailed descriptions on how to insert sgRNA sequences into this plasmid. growth strain : Egg cell-specific promoter-controlled expression of 3 FLAG-NLS-zCas9-NLS. lax wind forecastWebThe pHEE401E vector contains an antibiotic resistance gene, Hygromycin, which allows for this selection. Seeds which are able to grow healthy on the antibiotic plates are chosen to be grown into the next generation. Arabidopsis thaliana Plant Genotyping lax wind directionWebNov 22, 2024 · The pHEE401E vector has a type IIS restriction-enzyme-recognition site for ligation [ 6 ]. The other is a pBAtC-tRNA vector that uses a polycistronic tRNA-gRNA expression system and has two AarI type IIS restriction enzyme sites. Originally, these two vectors could carry two sgRNAs. lax why lineWebpHEE401E (Plasmid #71287) Print Enlarge View all sequences Purpose Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for … Plasmid pHEE401 from Dr. Qi-Jun Chen's lab contains the inserts sgRNA scaffold … Plasmid pCBC-DT1T2 from Dr. Qi-Jun Chen's lab contains the insert T1, U626t … laxwineandspirits.comWebpHEE401E (~100 ng/μl) 2 μl 10× T4 DNA Ligase Buffer (NEB) 1.5 μl 10× BSA 1.5 μl BsaI (NE B) 1 μl T4 DNA Ligase (HC, NEB) 1 μl ddH 2 O 6 μl Total volume 15 μl 5. Transform E.coli … lax wifi sign inWebMar 15, 2024 · Then the fragment was ligated into the binary vector pHEE401E by the restriction-ligation system (Wang et al., 2015). Then, the recombinant plasmid pHEE401E … lax what to doWebSep 24, 2024 · The structure of CSE-653 vector designed to sequentially cut S653 codon twice. pHEE401E backbone was used to ubiquitously express two sgRNAs by U6-26 and U6-29 promoters, and Cas9 was driven by an ... katharine mcphee not ur girl
