Myotube fusion index analysis automated
WebApr 29, 2024 · Analysis on cellular morphology and myogenic purity was carried out besides determination of SA-β-galactosidase expression and cell cycle profile. Myoblast differentiation was quantitated by determining the fusion … WebMyoCount: a software tool for the automated quantification of myotube surface area and nuclear fusion index [version 1; referees: awaiting peer review] Wellcome Open Research 2024, 4:6
Myotube fusion index analysis automated
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WebMar 11, 2024 · Fusion index is calculated as the number of nuclei within MyHC+ area divided the number of total nuclei. 2.5. Calcium Imaging The calcium imaging protocol was adapted from [ 21 ]. Briefly, cells were loaded with FURA-2AM (Invitrogen, Waltham, MA, USA) in Hank’s Balanced Salt Solution (HBSS) for 30 min in the dark at 37 °C. WebSep 1, 2024 · The fusion index (FI) was calculated as the number of nuclei within the myotube staining area divided by the total number of nuclei and expressed as a percentage. After exclusion of myotubes touching the image borders, whole entire myotubes were finally used to extract size parameters, including myotube area, myotube width, and number of …
WebCalculation of fusionindex, maturation index anddensity forthe myotubes The fusion index was calculated as the ratio of the nuclei number in myocytes with two or more nuclei versus the total number of nuclei.40The maturation index was defined as myotubes having five or more nuclei.40Edges and regions which did not show good stamping or cell … WebReproducibility and sensitivity of the fully automated MyoScreen platform are highlighted in assays that quantitatively ... The fusion index (FI) was calculated as the number of nuclei within the ... including myotube area, myotube width, and number of nuclei per myotube. AChR Clustering Assay Myotubes were treated with 0.5 or 5 nM rat ...
WebJan 21, 2024 · We have developed MyoCount, an open-source program that runs via the freely available MATLAB Runtime and quantifies myotube surface area and NFI. MyoCount allows the user to adjust its parameters to account for differences in image quality, magnification and the colour channels used in generating the image. WebJan 21, 2024 · MyoCount allows the user to adjust its parameters to account for differences in image quality, magnification and the colour channels used in generating the image. MyoCount measures of myotube surface area and NFI were compared to the mean of measures performed by two blinded investigators using ImageJ software (surface area R …
WebFusion index is a method for determining muscle cell myotubule formation. Myotubules are multinucleated cells which occur from cells fusing together and are the precursor to muscle fibers. The fusion index is calculated by staining the fixed cell and determining the number of nuclei in a myotubule compared to total number of nuclei in the sample.
WebDec 16, 2024 · ViaFuse can detect the borders of myotubes and identify nuclear clumps which have been limitations of previous muscle-centric imaging software. The ViaFuse macros require little computer power or space to run and user inputs to the ViaFuse macros are minimal, thereby automating the analysis process in a quick, easy, and accurate fashion. grand hustle entertainment atlanta gaWebNational Center for Biotechnology Information grand hustle clothingWebFeb 15, 2011 · Methods: We have developed a simple method to quantify myotube formation using micrographs of Jenner–Giemsa-stained C2C12 cultures. Because myotubes are darkly stained by Jenner–Giemsa dyes, the extent of myotube formation correlates with an increase in pixels attributed to the darkest tones. grand hustle gang clothinghttp://libna.mntl.illinois.edu/pdf/publications/129_bajaj.pdf grand hustle mixtapegrand hustle gang phone numberWebJan 21, 2024 · It is often desirable to characterise the morphology of myogenic cultures. To achieve this, the surface area of myotubes is often quantified, along with the nuclear fusion index (NFI). Existing methods of such quantification are time-consuming and subject to error-prone human input. grand hustle new artistWebMyoCount: A software tool for the automated quantification of myotube surface area and nuclear fusion index [version 1; referees: 2 approved] David Murphy, Thomas Nicholson, Simon Jones, Mary O'Leary Inflammation and Ageing Immunology and Immunotherapy Research output: Contribution to journal › Article › peer-review 2 Citations (Scopus) chinese food agassiz bc