Human myotubes eps mtor
Web20 jan. 2024 · In contrast, human myotubes differentiated on the feeder cells exhibited round cylinder-like myotubular morphology embedded within surrounding feeders and displayed obvious contractile activity... Web5 nov. 2024 · Human muscle biopsies of the vastus lateralis muscle were obtained from young, healthy, and sedentary men ( n = 3) as previously described ( Gnimassou et al., …
Human myotubes eps mtor
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Web19 jun. 2015 · To confirm that the EPS-induced contraction of C2C12 myotubes is an exercise-like condition, we examined AMPK phosphorylation and secretion of the myokines IL-6, MCP-1 and KC, the functional... Web28 jul. 2024 · Human myotubes from four donors were exposed to electrical pulse stimulation (EPS) or control for 48 h after which gene expression was profiled. Contributor(s) Mengeste AM, Nikolic N, Fernandez AD, Feng YZ, Nyman TA, Kersten S, Haugen F, Kase ET, Aas V, Rustan AC, Thoresen GH Citation(s) 35874526 Submission date Apr 22, …
Web25 jun. 2011 · The actions of exogenous leucine on mTOR signalling and amino acid transporters in human myotubes mTOR signalling is transiently activated by leucine within human myotubes independently of insulin stimulation. While this occurred in the absence of changes in gene expression of amino acid transporters, protein expression of hVps34 … WebMethods: Electrical pulse stimulation (EPS) was applied to adherent human myotubes. Cellular contents of ATP, phosphocreatine (PCr) and lactate were determined. Glucose …
WebElectrical pulse stimulation (EPS) is an in vitro method of inducing contractions in cultured skeletal muscle cells of human and animal origin. Motor neuron activation of muscle … Web6 aug. 2024 · Adherent human myotubes were either innervated with rat spinal cord segments or exposed to EPS. The expression pattern of MyHCs was assessed by …
Web28 jun. 2024 · In vitro models of muscle aging are useful for understanding mechanisms of age-related muscle loss and aiding the development of targeted therapies. To investigate mechanisms of age-related muscle loss in vitro utilizing ex vivo human serum, fasted blood samples were obtained from four old (72 ± 1 yr) and four young (26 ± 3 yr) men. Older …
Web14 dec. 2015 · Fig 1 shows ROS/RNS generation after EPS in C2C12 myotubes. An EPS of 40 V for 3 h significantly increased DCF intensity in the myotubes, compared with in nonstimulated (NS) controls (40 V: 8.1 ± 0.39, NS: 6.5 ± 0.23, Fig 1a and 1b). However, an EPS for 14 V or 20 V did not induce ROS/RNS production in C2C12 myotubes (Fig 1a … jcpenney delivery hoursWebskeletal muscle cells of human and animal origin. Motor neuron activation of muscle fibres can be replaced by applying EPS on differentiated skeletal muscle cells (myotubes) in culture [1,2]. Here we describe two protocols for EPS … jcpenney diamond studsWeb23 sep. 2024 · At this point, myotubes were nutrient deprived in Earl's Balanced Salt Solution (EBSS, ThermoFisher Scientific) for ∼14 h, with a subset of myotubes maintained in DMEM (2%HS, 1%PS) to serve as a ‘baseline’ condition. jcpenney diamond earrings on saleWebAdherent human myotubes were either innervated with rat spinal cord segments or exposed to EPS. The expression pattern of MyHCs was assessed by quantitative polymerase … jcpenney dickson city hoursWeb7 aug. 2013 · We applied EPS to human myotubes with parameters similar to the WB-EMS training (85 Hz, 15 V, 400 µs band width, 6 s stimulation, 4 s rest; 2 x 20 min on two consecutive days), and could show... lutheran missouri synod baptismWeb1 nov. 2024 · Myoblasts obtained from human muscle biopsies (n = 5) were differentiated into multinucleated myotubes and exposed to 8 h of EPS consisting of 2 ms pulses at … jcpenney dickson cityWeb22 mrt. 2012 · Electrical pulse stimulation (EPS) was applied to adherent human myotubes. Cellular contents of ATP, phosphocreatine (PCr) and lactate were determined. Glucose and oleic acid metabolism were studied using radio-labeled substrates, and gene expression was analyzed using real-time RT-PCR. jcpenney diamond drop earrings