site stats

Huh7 transwell

WebFurthermore, wound healing assay and transwell assay revealed that knockdown of STK39 repressed SK-HEP-1 and Huh7 cells migration and invasion. Interestingly, knockdown of … WebTranswell assay detecting migration capacity of huh7 cells (A: mir-nc group; B: mir-122 group; C: anti-mir-nc group; D: anti-mir-122 group; E: mir-122 + vector group; F: mir-122 …

PSPH induces cell autophagy and promotes cell …

Web30 sep. 2014 · HepG2 and Huh7 cells were incubated with 100 ng/ml RANKL or PBS as control for 24 h. qRT-PCR and Western blot analyses indicated that RANKL significantly promoted the expression of RANK. C. HepG2 and Huh7 cells were incubated with 100 ng/ml RANKL or PBS as control for 24 h. WebTumor heterogeneity dominates tumor biological behavior and shapes the tumor microenvironment. However, the mechanisms of tumor genetic features modul… senolytic drugs ingredients https://xhotic.com

[Effectiveness of TRB3 on human hepatocellular carcinoma cells

Web(A-D) Were the migration area of Huh7 and Hccm cells treated with atractylodin for 24 hours. (E-H) Were used to detect the number of invasive Huh7 and Hccm cells treated with atractylodin for 24 hours by Transwell assay, use crystal violet staining. Data are expressed as mean of three trials ± SD. Web1 aug. 2014 · Huh7 cells were stably transfected with infrared fluorescent protein [ 20 ]. Briefly, 293 T cells were transfected with pCMV-ΔR8.91, pMD.G, and pLKO AS3-reporter … Web1 dec. 2024 · Transwell analysis. Transwell chambers (8-μm pore size; Corning Inc., Corning, NY, USA) were used to measure HUVEC migration. In a 24-well culture plates, 500 μL RPMI-1640 Medium with 10% FBS was added into each lower chamber. HUVECs (1 ×10 4 cells/well) in 200 μL RPMI-1640 medium without FBS were seeded into each upper … senolytics arthritis

学会这 5 步，轻松搞定 transwell 实验 - 实验方法 - 丁香通

Category:合肥知恩生物 - 用心为客户提供满意的生物实验外包服务

Tags:Huh7 transwell

Huh7 transwell

High STRN Expression Promotes HCC Invasion and Migration …

Web6 sep. 2024 · Hep3B and Huh7 cells were placed in a 96-well plate, incubated at 37℃, and their absorbance wavelength were measured at 450 nm for 24, 48, and 72 h, respectively. Real time quantitative reverse transcription-polymerase chain reaction (RT-PCR) Web10 apr. 2024 · (D,E) Huh7 cells were transfected with ME2 siRNA (D) or stably infected with retroviral vectors expressing ME2 (E). (F) Huh7 cells were stably over-expressed ME2 or vector control. Migration was assessed by transwell. Numbers of migrated cells in the bar graph were quantified (top).

Huh7 transwell

Did you know?

Web19 jan. 2024 · Huh7 and LM3 HCC cell lines were transfected with two puro plasmid expressing sh-Tdo2 and scrambled control using transfection reagent (provided by Haro Life, Shanghai, China). Two shRNAs were designed to … Web所谓Transwell侵袭实验，其实是指将Transwell这一技术应用于肿瘤细胞侵袭研究的一种实验。 由于其简单易行、重复性好，因而得到了越来越广泛的应用，但不能认为研究肿瘤侵袭只有Transwell一种方法。 第二节 Transwell侵袭实验 我的课题涉及Transwell侵袭实验和Transwell迁移实验，其他方面的Transwell应用我不太清楚，因此这里主要谈 …

Web摘要： 研究背景原发性肝癌在我国发病率较高,其死亡率居肿瘤疾病别死亡率第二位.研究报道,包括我国在内的东亚地区肝癌危险因素主要是乙型肝炎病毒感染及存在于环境或食物中的黄曲霉毒素b1暴露.近年的研究发现,遗传,环境以及饮食等因素造成的肝脏铁过负荷是原发性肝癌的独立危险因素.由于 ... WebThe data of wound-healing assay, transwell migration assay and transwell invasion assay, showed that low concentrations of teroxirone suppressed migration and invasion determination of Huh7 cells.

WebThe LX-2 human hepatic stellate cell line has been extensively characterized and retain key features of hepatic stellate cytokine signaling, neuronal gene expression, retinoid metabolism, and fibrogen. LX-2 Human Hepatic Stellate Cell Line MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. WebThe program returns the range of each ORF, along with its protein translation. Use ORF finder to search newly sequenced DNA for potential protein encoding segments, verify predicted protein using newly developed SMART BLAST or regular BLASTP. This web version of the ORF finder is limited to the subrange of the query sequence up to 50 kb long.

Web11 apr. 2024 · Transwell TM and scratch migration assays were used to assess esculetin’s effects on the migration of HUH7 and HCCLM3 cells. According to the results, esculetin suppressed HUH7 and HCCLM3 cells migration (Figure 1D–G) in a dose-related manner. The outcomes show that esculetin could prevent HUH7 and HCCLM3 cells’ proliferation …

WebCCK-8 assay and transwell assay were used to examine the cell proliferation and invasion in Huh7 cells after treatment with PAB and a Notch inhibitor DAPT. Moreover, ... Results. The proliferation rate of Huh7 cells exposed to PAB of 0.5, 1, 2, 4, 8, 10, 20, 40, 80, 100, and 200 μmol/L revealed a time-and dose-dependent decrease in vitro, ... senolytics ipoWebJouan等人发现HuH-7细胞上调了一些与法尼醇X受体（FXR）和核因子红细胞2相关因子2（Nrf2）相关的转运蛋白。 这些数据表明，在表达一些主要的肝脏药物转运体时，它可能被用来研究药物与MRP的相互作用。 应用：人肝癌细胞系Huh7的基因组特征对传代次数非常敏感，分化后的Huh7细胞可能适合于研究药物代谢酶蛋白表达的肝毒性作用，Huh-7细胞 … senolytics companiesWeb12 apr. 2024 · Hepatocellular carcinoma (HCC) is one of the most common types of primary liver cancer and is the third leading cause of cancer-related deaths worldwide [1]. The 5-year relative survival rate for patients with HCC is only 18%, indicating the … senolytics 2022http://www.immocell.com/ryxbx/1717.html senolytics benefitsWebTranswell 小室在细胞实验中很常用，共 培养 实验、趋化实验、细胞迁移、细胞侵袭以及药物转运实验都会用到，但小室的规格有很多种，不同的实验需要用到的小室规格也不同。 根据 transwell 板的不同，transwell 小室可以分为 6 孔板小室、12 孔板小室和 24 孔板小室以及和 transwell 皿配套的 75 mm 直径的小室；根据孔径的不同，从小孔径到大孔径又分 … senolytics for saleWebMethods: The human hepatocyte cell line (HuH7) and HSC cells (LX2) were exposed to FFA for 24 h in 3 different experimental set-ups: (A) monoculture of HSC; (B) Transwell® … senolytics manufacturerWebTranswell assays of Huh7 and HCCLM3 cells transfected with control and ATAD2-RNAi-lentivirus. (A) ATAD2 depletion had a measurable inhibitory effect on cell invasion in both … senolytics ipf