WebAug 18, 2024 · Apoptosis Analysis. The percentage of apoptotic cells was evaluated by the Guava Nexin Assay. Briefly, the percentage of live, apoptotic and necrotic cells was assessed using the Guava Nexin Reagent (containing 7-AAD and Annexin-V-PE) and analyzed by Guava Nexin software. WebNov 27, 2024 · 632844 The Guava Nexin Reagent is a mix-and-read assay for monitoring externalization of phosphatidylserine (PS) through the binding of Annexin V to the exposed PS. I have used this reagent for measuring levels of Annexin V in our control and test samples. Our results was good and showed difference between the group.
Guava Nexin Assay Kit Millipore Bioz
WebOct 15, 2008 · Cell proliferation assay. The in vitro cytotoxicity was determined using 3- (4,5-dimethylthiazol-2-yl)-5- (3-carboxymethoxyphenyl)-2- (4-sulfophenyl)-2H-tetrazolium (MTS), which is chemically reduced by metabolically active cells into formazan, and the intensity of the resulting color is proportional to the number of living cells. CDC. WebDec 1, 2024 · Guava Nexin assay (4500-0450, Guava Technologies, CA, USA) was utilized for measuring HaCaT cell apoptosis as earlier described [22]. Briefly, cells were cultivated into 6-well plate and subjected to relevant treatment. fhaw airport code
In vitro - American Association for Cancer Research
WebJun 23, 2024 · MTT assay showed that ABT199 + AZD5 displayed a similar synergistic effect on H1975AR compared with ABT263 + AZD5. Flow cytometry analyses showed that ABT263 + AZD5 had higher cell populations of early apoptotic and apoptotic in Nexin assay as well as higher cell population arrested at pre-G1 phase representing apoptotic cells … WebNov 1, 2013 · The impact of geranylgeraniol and lovastatin (Lova) on the initiation of apoptosis in human DU145 prostate carcinoma cells. (a) DU145 cells were incubated with geranylgeraniol (0, 50, and 100 μmol/L) and lovastatin (10 μmol/L) for 24 h and then analyzed by flow cytometry using the Guava Nexin ™ Assay. Cells in the lower left … WebGuava® Cell Cycle Reagent To investigate cell cycle phases, the Guava® Cell Cycle Reagent can be used for measuring G0/G1, S, and G2/M phase distributions. department of communications org chart